Analyzing the effects of DMARD treatment on genetic expression

Anna Boateng (s175562), Marlies Goedknegt (s220675), Anne Gjelstrup (s194530), Katja Jagd (s185395), Olivia Ronnenberg (s183359)

Introduction

Genome-wide transcriptional effects in RA (rheumatoid arthritis) patients, undergoing treatment with several disease-modifying drugs (DMARDs).

Purpose: What are the genetic differences in RA patients, before and after DMARD treatment?

Materials and methods

Results: Differential expressed regulators

Does DMARD treatment affect expression levels?

Results: Differential expressed regulators

What are the differences in genetic expression levels in RA patients, compared to healthy control samples?

Results: Differential expressed regulators due to treatment

What are the differences in genetic expression levels in RA patients, before and after DMARD treatment?

Results: Target molecules

What types of biological molecules are the common upstream regulators?

Results: Target molecules

How does the treatment change the influence of the upstream regulators?

Results: Molecular mechanisms

Which modules are the target molecules involved in?

Discussion

  • Clearer purpose at the start
  • Exclude data
  • Iterate the process
  • Look into pathways